Retract and activate the biofilter

The problem

There is a fundamental problem with biofilters in aquaponics: If you put fish in a new system, there are no bacterial carpets on the substrate that can clean the water of ammonia (from the fish's excretions). Without this cleaning, the fish poison themselves after a while. But without fish, bacteria do not form because they cannot find food (ammonia) in the clean water.

 

The solution

Before fish are introduced into the system, ammonia is added in a controlled manner. For this it is sufficient:

  • Test strips for the pH value (sourced from the aquarium store)
  • Test for ammonia content of the water (sourced from the aquarium store)
  • Ammonia solution (pharmacy)
  • Vinegar if the water becomes too alkaline (supermarket)
 

Regarding Amonik: Please be sure to wear protective gloves, respiratory protection and safety glasses when using it.

Carrier for biofilters

Most biofilters use media such as sand, gravel, river gravel, or some form of plastic or ceramic material in the form of small beads and rings.

When operating a biofilter, the main problem is to prevent the filter material from drying out or becoming waterlogged in places and thereby enable an even flow through the filter bed. This can be achieved primarily by encapsulating the biofilters. The disadvantages of these systems are often the large space requirement, the cost-intensive fan energy to increase the pressure and the constant irrigation. Compared to other processes, such as ionization with ionization tubes, the constant biological cleaning process is often advantageous due to CO2 savings and numerous economic aspects, such as average acquisition costs, long-term filter service life and average operating costs. ssa biofilter medium

Commercial Biofilter Media (SSA: Specific Surface Area): (A) K1, K3, (B) Atlantic Bio-Balls, (C) Honeycomb Bio-Balls, and (D) MB3 Media.

Trickle filter transitions

Schematic cross section of the contact area  of ​​the bed medium in a trickling filter.

 

Procedure (according to Bernstein, 2011)

  • Plant the system.
  • Add ammonia until 2-4 ppm is reached. Note the quantity used. For my tank (approx. 600 l) you need about 75ml of 25% ammonia solution.
  • This amount is added daily until at least 0.5 ppm nitrite can be measured in the water. If the ammonia level approaches 8 ppm, wait until it drops back to 2-4 ppm before adding more ammonia. Since the increase can happen quite quickly, a higher dosage is not advisable.
  • As soon as the nitrite appears in the detection, the ammonia doses are halved. If the nitrite level goes above 5 ppm, ammonia additions should stop until the level drops to 2 ppm.
  • Once the nitrate reaches 5-10 ppm, wait until the nitrite and ammonia levels are at zero. Then you can add fish.
  • The pH value should be 6.8 to 7.0 and can be adjusted carefully with vinegar (too high a value) or calcium carbonate/soda (too low a value), of course before the fish are added.

 

What happens in the biofilter?

By adding ammonia, bacteria find food and convert ammonia into nitrite. This nitrite then serves as an energy source for other microorganisms. The oxidation of nitrite (NO2) produces nitrate (NO3). This process is the second step in the nitrification of ammonia (NH3) to nitrate. The nitrate then serves as fertilizer for the plants.

  

The nitrification process in aquaponics

Ammonia ⇒ Nitrite ⇒ Nitrate
The nitrifying bacteria play an important role in an aquaponics system. They convert fish waste so that ammonia enters the system as nitrate. Nitrification in aquaponics is a two-step process and involves two nitrifying bacteria:

1. Conversion of Ammonia to Nitrites – This is carried out by the Nitrosomonas when there is an overload of food waste, it produces excess ammonia in the water. The ammonia must be removed to keep the fish healthy. Nitrosomonas bacteria convert the ammonia into nitrites.

2. Conversion of nitrites to nitrate – This is carried out by Nitrobacter . Nitrobacter bacteria feed on nitrites. The nitrites are converted into nitrates once the nitrites are consumed by Nitrobacter. Plants grow quickly when they absorb nitrates. Excessive nitrites can kill the fish. In order to keep the fish and plants healthy, nitrites must be converted into nitrates.

Nitrifying bacteria multiply slowly and form colonies; It can take days, weeks, or even months. Nitrifying bacteria require a dark location, good water quality, and sufficient food and oxygen to colonize. There are five key parameters to support nitrifying bacteria. If these parameters are met, it can be assumed that the bacteria are present and functioning well.

 

1. Large surface area

Biofiltration with a high specific surface area is important to develop extensive colonies of nitrifying bacteria. There are many materials that can be used in aquaponics, either as growing media or for biofiltration. Volcanic gravel, expanded clay pebbles, commercial plastic biofilter balls, and plant roots all act as a surface for the bacteria to live on. The smaller and more porous the particles, the larger the surface area available for bacteria to colonize, resulting in more efficient biofiltration.

 

2. Water pH

Nitrifying bacteria function properly when the pH is between 6 and 8.5. The ideal pH in aquaponics is usually 6-7, which is a compromise between all organisms in the system.

 

3. Water temperature

The ideal temperature range for the bacteria is between 17° Celsius to 34° Celsius (~ 63°F - 93°F). This area promotes the growth and productivity of bacteria. If the water temperature drops below this range, it reduces the productivity of the bacteria. If it rises far above this (42 °C), the bacteria die.

 

4. Dissolved oxygen

Nitrifying bacteria require sufficient levels of dissolved oxygen in the water to grow healthily and maintain productivity. The optimal dissolved oxygen level is 4 - 8 ppm, nitrification does not occur if the dissolved oxygen concentration falls below 7.0 ppm. You can ensure adequate biofiltration and dissolved oxygen by adding aeration, either using air stones or through flood-and-drain cycles in media beds.

 

5. No UV light

Nitrifying bacteria are sensitive to light until they are fully established. and sunlight can damage the biofilter. Media beds protect the bacteria from sunlight, but if you use a biofilter, protect it from direct sunlight.


Undesirable Bacteria
Nitrifying and mineralizing bacteria are important and useful for aquaponics, but there are some types of bacteria that are harmful to an aquaponics system, these are:

 

1.Sulphate reducing bacteria

These bacteria are often found in anaerobic conditions and smell like rotten eggs. These bacteria are gray-black in color and only grow under anoxic conditions. It is important to provide adequate ventilation and increase mechanical filtration to prevent the accumulation of these bacteria.

 

2. Denitrifying bacteria

These bacteria also thrive in anaerobic conditions and are responsible for denitrification. They convert nitrites back into atmospheric nitrogen, which is not available to plants. In aquaponics systems, these bacteria can reduce efficiency by removing the nitrogen fertilizer.

 

3. Pathogenic bacteria

These bacteria can cause diseases in plants for fish and humans. It is important to have good practices ( CMMI ) to minimize the risk of disease in an aquaponics system. You can prevent pathogens from entering the system by keeping all other animals (pets, farm animals, etc.) away from your system. Placing your aquaponics system in a closed greenhouse can also prevent pathogenic bacteria get into your system.

 

System Cycling and Creating a Biofilter Colony
System cycling in aquaponics refers to creating a healthy bacterial colony when you start your new aquaponics system. The process takes place once a new aquaponics system is built and typically takes 4 weeks to two years. The process involves introducing a source of ammonia (usually fish) into a new aquaponics system, feeding the new bacterial colony, and building the biofilter (by the bacteria themselves). Progress is measured by monitoring nitrogen levels.

Without bacteria, the nitrogen cycle does not take place. The nitrogen cycle converts the ammonia from fish waste into nutrient-rich fertilizer for plants. The nitrogen cycle only occurs when the nitrifying bacteria are present. In order for this to take place, ammonia must be added to the system. This ammonia can be added with the fish or with water from another aquaponics system where the bacterial colony is already established. When more ammonia is added, more bacteria are produced, making the system work more efficiently. Once ammonia-converting bacteria become established, they produce nitrites, which allows the bacteria to use the nitrites and produce nitrates from them. A system is fully established (broken in) once ammonia or nitrites are measurable during the test.

 
Fish without a bike

This process is often used in new aquaponics systems or tanks because it can be done without concerns about fish safety. To begin fishless cycling, you need to introduce ammonia into the system.

The process is simple; After the system is set up, begin adding the ammonia solution to the water. Once the system (tank, pumps, etc.) has been completely circulated, you should have achieved at least a value of 0.2 ppm.

 

Ways to Reduce System Cycle Time
System cycle is a very slow process. Depending on the size and type of system, the process can take up to 18 months. However, there are other ways to set up the system faster. One method is to use water from another aquaponics system where the bacterial colony is already established. It is helpful to pass part of the biofilter as a bacterial strain to a new aquaponics system. This reduces the time required to go through the system. Some prefer to add a little urea or a dead fish to the tank to start the decomposition process. But pay attention to the formation of pathogenic bacteria - you must avoid these. Household ammonia can also be used. However, make sure the product is 100 percent ammonia and does not contain other ingredients such as detergents or heavy metals that could damage the entire system.

Once the ammonia and nitrite levels are below 1 ppm you can add plants and fish to the system. Start with a few fish and closely monitor nitrogen levels. Be prepared to replace the water if ammonia or nitrite levels rise above 1 ppm while the system continues to run.

 

Conclusion
Bacteria are the little microscopic creatures that do all the work in an aquaponics system by converting fish waste into nutrients that the plants can absorb. Without them, the system would fail, the fish would die and the plants would not grow or die completely. Bacteria are just as important in an aquaponics system as the fish and the plants. 


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