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Nitrogen Balance in Aquaponics - Calculation and Conversion

Nitrogen Balance in Aquaponics: From Ammonium to Plant Nutrition

Question: If a system produces 6.49 g NH₄⁺/day, how much of it is converted to nitrate for the plants or remains?

 

1. Basic Nitrification Process

In a well-functioning biofilter, the following reaction takes place:

  1. Step 1: NH₄⁺ → NO₂⁻ (nitrite) by Nitrosomonas bacteria
  2. Step 2: NO₂⁻ → NO₃⁻ (nitrate) by Nitrobacter bacteria

Overall Reaction:

NH 4 + + 2 O 2 NO 3 - + 2 H + + H 2 O

 

2. Conversion for 6.49 g NH₄⁺/Day

A) Conversion to NH₄-N (Nitrogen Basis)

Since plants and bacteria utilize nitrogen, we convert to an N-basis:

NH₄-N = NH 4 + 1.288 = 6.49 1.288 = 5.04 g N/Day

 

B) Theoretical Nitrate Production (100% Efficiency)

With ideal nitrification:

NO 3 - produced = NH₄-N × 4.426 = 5.04 × 4.426 = 22.31 g NO₃⁻/Day

Or on a nitrogen basis:

NO₃-N = NH₄-N = 5.04 g N/Day

 

3. Realistic Estimates (Practical Factors)

Factor 1: Biofilter Efficiency

  • Good Biofilter: 85-95% conversion
  • New/Uneven Biofilter: 70-85%
  • Assumed 90% Efficiency:
Available NO₃-N = 5.04 × 0.90 = 4.54 g N/Day
 
Available NO₃⁻ = 4.54 × 4.426 = 20.08 g NO₃⁻/Day

 

Factor 2: Plant Uptake

  • Typical Uptake Efficiency: 60-80% of available nitrate
  • Plants use N primarily as NO₃⁻, but also some NH₄⁺ (approx. 10-20%)
  • Assumed 70% Uptake:
Plant-N-Uptake = 4.54 × 0.70 = 3.18 g N/Day

Of this as nitrate: ≈ 2.86 g N/Day (90% of 3.18)

 

Factor 3: Other Losses/Processes

  1. Denitrification (anaerobic zones): 5-15% N loss as N₂ gas
  2. Algae/Biofilm Growth: 5-10%
  3. NH₃ Volatilization: 1-5% (pH dependent)
  4. Sludge/Sediment: 2-8%

 

4. Practical Balance for 6.49 g NH₄⁺/Day

ProcessN-Share (g/Day)% of InputRemark
Input: NH₄⁺ from Fish 5.04 g N 100% 6.49 g NH₄⁺
Nitrification (90%) 4.54 g N 90% Converted to NO₃⁻
Plant Uptake (70%) 3.18 g N 63% Actually utilized
Denitrification (10%) 0.50 g N 10% Loss as N₂
Algae/Biofilm (5%) 0.25 g N 5% Unwanted growth
NH₃ Volatilization (2%) 0.10 g N 2% pH/temp dependent
Accumulation in System 1.01 g N 20% Increasing nitrate concentration

 

5. What This Means for Your Plants

A) Nitrate Availability Per Day:

Available NO₃⁻ 20.08 g/Day

 

B) Amount of Plants That Can Be Supported:

Lettuce: Requires ≈ 0.15 g N/plant/growth period (6 weeks)

→ Daily requirement: ≈ 0.0036 g N/plant/day

Possible Number of Plants:

3.18 0.0036 880 lettuce plants

Tomatoes: Require ≈ 3.5 g N/plant/season

→ Daily requirement: ≈ 0.028 g N/plant/day (at 125 days)

Possible Number of Plants:

3.18 0.028 114 tomato plants

 

6. Important Parameters

Recommended Calculation Steps:

  1. NH₄⁺ Input → Convert to NH₄-N
  2. Apply biofilter efficiency (user can set: 70-95%)
  3. Plant uptake efficiency (60-80%)
  4. Subtract losses (denitrification, algae, etc.)
  5. Calculate Nitrate Accumulation:
Nitrate Accumulation = NH₄-N Input - Plant Uptake - Losses

Warning at accumulation > 50 mg/L NO₃-N (approx. 220 mg/L NO₃⁻)

 

Summary

From 6.49 g NH₄⁺/Day you obtain about 3.2 g N/Day for your plants, which corresponds to approx. 20 g NO₃⁻/Day. The actual amount depends heavily on biofilter efficiency and plant uptake!


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